-- 作者:admin
-- 发布时间:9/23/2004 2:05:00 AM
-- about bioinformatics
about bioinformatics 发信人: kingsyl (阅读文献中), 信区: Bioinformatics 标 题: about bioinformatics 发信站: 北大未名站 (2001年03月12日19:45:10 星期一), 站内信件 Bioinformatics has two main tasks in the Protein Structure Factory: To predi ct what can be done and to propose what should be done. Predicting what can be done is equivalent to identifying proteins that will permit their three-d imensional structures to be determined by X-ray crystallography or NMR spect roscopy. These proteins will have some properties in common. They will be so luble in aqueous buffers up to a critical concentration, they will have a de fined globular structure, and this structure will be stable for at least as long as it takes to grow and expose crystals or to measure the NMR spectra. Proteins that contain long stretches of hydrophobic or charged amino-acid re sidues, have extended sequence repeats or use a limited repertoire of amino acids over long polypeptide segments often do not display these properties. However, they may still contain single or multiple domains that permit struc ture analysis. In addition, bioinformatics will provide valuable information aiding the structure determination by predicting sites of post-translationa l modification and identifying proteins of known, similar tertiary structure . Structural prediction will be used to decide whether a given protein will be studied by NMR spectroscopy or by X-ray diffraction or, for the latter ca se, whether its structure analysis will require experimental phase determina tion or can be based on a homologous model. To propose what should be done is the more challenging task. It is equivalen t to finding proteins with interesting properties such as novel folds or a f unction in biochemical pathways that may be associated with disease. The mor e interesting a protein appears, the more effort will have to be invested in its structure analysis. Computational tools for functional sequence assignm ents are currently being developed21. This work addresses questions concerni ng the subcellular localization of proteins, their membership in families de fined by function22-24 and their involvement in pathological states25 -- 路边的野花不能采?:)- 斜阳尽处是春山,行人更在春山外! ※ 来源:·北大未名站 bbs.pku.edu.cn·[FROM: 162.105.45.137]
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